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. 2003 Oct;23(19):6713–6724. doi: 10.1128/MCB.23.19.6713-6724.2003

FIG. 2.

FIG. 2.

Identification of PDX-1 binding sites in the human LRH-1 promoter. (A) Computational analysis of a ±2-kb fragment of the regulatory region of the human LRH-1 gene demonstrating the presence of five potential binding sites for PDX-1 (Pdx 1 to 5) homologous to the PDX-1 consensus binding site present in the rat insulin promoter. In the 293T and MiaPaca-2 cell lines, cotransfection of an expression vector encoding murine PDX-1 and a luciferase reporter containing ±2 kb of the human LRH-1 promoter induces human LRH-1 promoter activity compared to an empty expression vector. (B) Alignment of ±500 bp of the human, mouse, and rat LRH-1 promoters. The highly conserved site 5 is indicated in gray. Sequence differences are boxed. Transcription initiation sites are indicated by arrows.