FIG. 6.

Bur1 cross-linking is reduced at the 3′ end of genes. ChIPs were performed with an HA₃-tagged Bur1 strain (YSB770). Immunoprecipitation was carried out with anti-Rpb3 (a polymerase II subunit) and anti-HA recognizing the tagged Bur1. Various regions of the constitutively expressed genes PMA1, ADH1, PYK1, and YEF3 were examined (locations of the primer pairs are depicted in the bottom panel). Quantitation of the PCR results after normalization to input is graphed below the gels. The numbers (y axis) designate the signal of the specific primer pair product relative to the nontranscribed no-ORF negative internal control.