FIG. 7.

Bur1 mutants do not affect association with transcription complexes. (A) Epitope-tagged Bur1 mutant alleles were transformed into strain YSB787, and the resulting strains were used for ChIP analysis in the presence of untagged wild-type Bur1. Cross-linking of each mutant to promoter and coding sequences of the PMA1 and ADH1 genes is shown. (B) The Bur1 T240A allele shows a similar cross-linking pattern to wild-type Bur1 throughout the PMA1 gene, indicating that phosphorylation of the Bur1 T-loop is not the signal for disengagement at the 3′ untranslated region (UTR). Note that the T240 mutant strain does not contain wild-type Bur1.