TABLE 1.
RT-PCR primers
| mRNA | DNA length (bp) | Primer set (forward, reverse)a |
|---|---|---|
| α-Globin | 315 | 5′-GTGGACGACATGCCCAACGC-3′, 5′-CCCACTCAGACTTTATTCAA-3′ |
| γ-Globin | 279 | 5′-AAGGTGCTGACTTCCTTGGG-3′, 5′-ATCCTTGAAAGCTCTGCATC-3′ (49) |
| GAPDH | 180 | 5′-CAACTACATGGTTTACATGTTC-3′, 5′-GCCAGTGGACTCCACGAC-3′ |
| SAP49 | 270 | 5′-AACCGCTGCTGTGGGAACTGTTTC-3′, 5′-GCAACTTCTCATCAATCTCAGGGTC-3′ |
| SAP62 | 361 | 5′-CACTTCACAACAATGAGGGGAGC-3′, 5′-AAGGCAATGGTCTCGTAGGGTTCG-3′ |
| Trx2 | 239 | 5′-TTGGCTGACAAGCAGGGATGAG-3′, 5′-AAAGGCGTATGGGAGGGAAGAC-3′ |
| αCP2,b αCP2-KL | 360, 330, 237 | 5′-GGGGTACCTGTTCTAGCTGCWCCCCAT-3′ (RT), 5′-CGTGACCATYCCGTACC-3′, 5′-TTTGGAATGGTGAGTTCATG-3′ (29) |
| C-Src | 328 | 5′-GGAACAAAGTCGCCGTCAAGTG-3′, 5′-TCCTCAGACACCAGCACATTGC-3′ |
| JunD | 230 | 5′-GAGAAGAACAGAGTGTTCGAT-3′, 5′-ACAGGAATGTGGACTCGTAGC-3′ |
| A-Raf-1 | 113 | 5′-AAAGTATACCTGCCCAACAA-3′, 5′-CAGCAGTCCTGATTTAGACC-3′ |
| Cln2 | 267 | 5′-TCCCCACTGCTACTACCTTA-3′, 5′-CAAGAGTGAGAGTTCCTTGG-3′ |
| PPT2 | 215 | 5′-CCTCTCCACAGATGGGACAGTATG-3′, 5′-GGCATTGGGATGGTCTCTTTCC-3′ |
| coxll | 106 | 5′-GCAAACCACAGTTTCATGCC-3′, 5′-GGCTCTAGAGGGGGTAGAGG-3′ (49) |
a
In all reactions except for αCP2 amplifications, the reverse primer was used for RT as well as the PCR.
b
The RT primer is indicated (RT) followed by the forward and reverse primers.