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. 2003 Oct;23(19):6759–6768. doi: 10.1128/MCB.23.19.6759-6768.2003

FIG. 4.

FIG. 4.

Functions of the two RSF subunits. (A) ATPase activity. The graph shows the quantification of phosphate released during the ATPase reaction. Lanes show the following: no factor added (lane 1), increasing amounts of recombinant hSNF2H (between 0.2 and 0.9 μg, lanes 2 to 4), increasing amounts of recombinant Rsf-1 (0.2 and 0.4 μg, lanes 5 and 6, respectively), and the same amount of recombinant hSNF2H used in lane 3 plus increasing amounts of Rsf-1 (0.2 and 0.4 μg, lanes 7 and 8, respectively). (B) Chromatin assembly. An agarose gel shows a micrococcal nuclease digestion of chromatin assembly reactions carried out without factor (lane 1), with recombinant RSF obtained by coexpression of the subunits (lane 2), with recombinant Rsf-1 plus recombinant hSNF2H (lane 3), with recombinant hSNF2H alone (lane 4), and with recombinant Rsf-1 alone (lane 5).