FIG. 3.

Construction of an inversion chromosome inv(11) (Csn3-Zfp179). Csn3 and Zfp179 are ≈3 Mb apart on mouse chromosome 11. They were targeted with the 3′ HPRT and 5′ HPRT vectors, respectively, which resulted in the duplication of exons III to VI in Csn3, duplication of exons I to XV in Zfp179, and insertion of the entire vector (42). After the introduction of Cre recombinase, the chromosome region between two LoxP sites was inverted, which was confirmed by Southern analysis with SacI and a probe from Zfp179, depicted as solid horizontal bars. P, puromycin resistance gene; N, neomycin resistance gene; WT, wild type; Inv, inversion.