FIG. 8.

Outgrowth of blastocysts in vitro. Morphologically abnormal blastocysts were not observed in 33 blastocysts. Wild-type blastocyst (A) and Csn3^−/− mutant blastocyst (B) are morphologically similar. After 3 days of culture, the inner cell mass (ICM) developed in both mutant and wild-type embryos (C and D). After 5 days of culture, the ICM of wild-type embryos developed more in comparison to the 3-day culture (E), while the ICM of mutant embryos in the 6-day culture appeared greatly reduced in cell number (F). TG, trophoblast giant cells. Magnification: 400× for blastocysts; 200× for cultures.