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. 2003 Oct;23(19):6750–6758. doi: 10.1128/MCB.23.19.6750-6758.2003

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FIG. 3. — The induction of direct-T₃-response genes is inhibited in transgenic animals expressing dnTR. Wild-type (lanes 1 and 2) or transgenic (lane 3) tadpoles at stage 54 were treated with 0 or 50 nM T₃ for 1 day. RT-PCR analysis was carried out for the expression of the indicated T₃-regulated genes on RNA isolated from the tail and intestine. Included in each reaction tube were a primer set for a T₃-regulated gene and one for the T₃-independent control gene rpl8 (57). The amplified DNA was analyzed by agarose gel electrophoresis. Note that TRβ and TH-induced bZip-containing transcription factor (TH/bZip) were induced both in the tails and intestines of wild-type tadpoles (compare lanes 1 and 2), as expected. Their induction was dramatically inhibited in the transgenic tadpoles expressing dnTR (lane 3).