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UNC-73B DH is an exchange factor for CeRAC and MIG-2. The normalized change in fluorescence (F) upon addition either of GST/UNC-73B DH/PH/SH3 to preloaded (mant-GDP) CeRAC (▪), MIG-2 (▾), CeRHO (□), and CeCDC42 (•) or of GST to preloaded CeRAC (▴) and MIG-2 (⧫) is shown. Proper loading of mant-GDP to CeRHO and CeCDC42 was demonstrated by monitoring release of the fluorescent nucleotide by EDTA (data not shown).
