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. 1998 Mar 3;95(5):2509–2514. doi: 10.1073/pnas.95.5.2509

Figure 3.

Figure 3

Generation of stable recombinants in bacterial cells. (A) DNA from recombinant pAdEasy-GFP+GAL constructs derived from homologous recombination of pAdTrack-CMV-βgal and pAdEasy-1in BJ5183 cells was purified from minipreps. The DNA was analyzed in supercoiled form by electrophoresis through an 0.8% agarose gel and ethidium bromide staining. Lane 1, pAdEasy-1 control; lane 2, pAdTrack-GFP+GAL control; lanes 3–12, different pAdEasy-GFP+GAL clones. Based on the migration rates, the clones in lanes 3, 4, 6, 8, 9, 11, and 12 were potential valid recombinants. (B) Representative digestions with BamHI (lanes 1–3), PacI (lanes 4–6), and SpeI (lanes 7–9). Plasmids pAdTrack-CMV (lanes 1, 4, and 7), pAdEasy-1 (lanes 2, 5, and 8) and a pAdEasy-GFP+GAL recombinant (lanes 3, 6, and 9) are shown. ∗ indicate the diagnostic fragments obtained with each enzyme.