FIG. 3.
LexADBD-Ada2β two-hybrid screen. (A) Test of LexADBD-Ada2β interaction with Baf57. Either LexADBD-Ada2β or LexADBD-Lamin was cotransformed into yeast with deletion mutants of Baf57 (dimensions are indicated in parentheses) fused to the VP16 activation domain. The strength and specificity of interactions were assessed by two means: (i) color LacZ assay on filters and (ii) ability to grow on 100 mM 3-AT. (B) Schematic of Baf57. Functional domains of Baf57 are indicated. The Ada2β-interacting region is shown: P-rich (proline-rich), HMG domain (high-mobility-group domain), Charged, Kinesin-like coiled-coil region, and Acidic region.