FIG. 3.
Anthranilate consumption (a) and catechol formation (b) by IPTG-induced E. coli strains carrying the andAcAd genes (pGJZ1365), the andAcAdAb genes (pGJZ1364), and the andAcAdAbAa genes (pGJZ1362 and pGJZ1363). pGJZ1362 has a putative membrane protein gene (ORFM) right after the andAcAdAbAa genes. The E. coli strain carrying pGJZ1361 was used as the negative control. Transformation of anthranilate to catechol was monitored by HPLC analysis. The initial concentration of anthranilate was 1.0 mM.