TABLE 1.

Plasmids and primers as well as bacterial strains and their susceptibilities to VGJ-Knφ

Strain, plasmid, or primer	Description	Source or referencea	Susceptibility to VGJ-Knφb
E. coli JM109	recA1 supE44 endA1 hsdR17 gyrA96 relA1 thiΔ(lac proAB)	Promega	NA
V. cholerae strains
569B	O1, Classical, Inaba	Calcutta, India, 1945	1.6 × 108
O395	O1, Classical, Ogawa	Calcutta, India, 1964	8.0 × 107
CA401	O1, Classical, Inaba	Calcutta, India, 1953	9.8 × 107
1395	O1, Classical, Inaba	CIEI	7.6 × 107
CA385	O1, Classical, Ogawa	Calcutta, India, 1953	1.8 × 103
NIH35A3	O1, Classical, Inaba	India, 1941	4.8 × 104
N16961	O1, El Tor, Inaba	Bangladesh, 1975	7.7 × 106
C7258	O1, El Tor, Ogawa	Peru, 1991	1.9 × 107
638	C7258 A(CTXφ prophage), hap::celA	3	NDc
C6706	O1, El Tor, Inaba	Peru, 1991	1.3 × 108
KHT46	C6706 Δmsh41	54	ND
KHT52	C6706 ΔtcpA10	54	2.1 × 108
1333	C6706 Δ(CTXφ prophage), hap::celA	CNIC	3.8 × 107
Peru-15	C6709 Δ(CTXφ/RSI prophages), recA::ctxB	32	5.4 × 107
E7946	O1, El Tor, Ogawa	Bahrain, 1978	9.2 × 106
Gamene	O1, El Tor, Ogawa	Institute Pasteur	5.0 × 106
3083	O1, El Tor, Ogawa	Viet Nam, 1964	2.1 × 106
Malawy	O1, El Tor, Inaba	Institute Pasteur	4.1 × 107
Lima	O1, El Tor, Inaba	Lima, Peru, 1991	8.3 × 106
SG25-1	O139	Calcutta, India, 1993	2.4 × 103
1837	O139	Bangladesh, 1993	7.6 × 106
CRC262	O139	Bangladesh, 2000	2.4 × 107
CRC266	O139	Bangladesh, 2000	4.7 × 107
MO45	O139	Madras, India, 1993	3.2 × 104
MO12C	O139	Madurai, India, 1993	7.2 × 104
Plasmids
pUC19	General purpose vector	59	NA
pJM132	Plasmid expressing wild-type mshA from C6706	17	NA
pKOR1	R6K origin of replication plus a Knr cassette from pUC4K	This study	NA
Primers
VGJphi1	5′-CAAGTCGAGACGAACTAAGC-3′	This study	NA
VGJphi2	5′-CCAAGTAATACGATAAACGC-3′	This study	NA
VGJphi3	5′-CGGCATTTTGTGGGCATCAG-3′	This study	NA
VGJphi4	5′-CGGCCTATGGTAAAAGTTGC-3′	This study	NA
VGJphi5	5′-TCTCTGGTATCACCGATGCG-3′	This study	NA
VGJphi6	5′-GCGCTTCCTCCCGTGTCCTC-3′	This study	NA
VGJphi7	5′-GCTATGGCTAGAATCAAAGGG-3′	This study	NA
VGJphi8	5′-AAGCTAAAGAGCAAGGCG-3′	This study	NA
NJ1	5′-GGATGTTTACGATAGC-3′	This study	NA
NJ2	5′-TAGAACGTGTCATTGCATCG-3′	This study	NA
NJ4	5′-GCACACAATTGACGTAAGTAC-3′	This study	NA

^aCIEI, Centro de Investigacion de Enfermedades Infecciosas, Cuernavaca, Mexico; CNIC, Centro Nacional de Investigaciones Científicas, Havana, Cuba.

^bNumber of Kan^r transductants appearing after infection of ∼10⁸ cells of the receptor strain with 10¹⁰ Kan^r transducing particles (multiplicity of infection, 100). NA, not applicable.

^cND, no Kan^r transductants detected.