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Effect of iron on hupA::luxAB (pHL02) expression in the wild type (AR) and in the rpoS mutant. Iron in the medium was depleted by adding 0.2 mM 2,2′-dipyridyl. The hupA::luxAB activities were normalized by dividing the number of relative light units (RLU) by the OD595. The hupA::luxAB activities of four independent cultures at the exponential phase (OD595 = ∼0.5) were averaged and are indicated, with their standard deviations.
