TABLE 1.
Strains, plasmids, and primers used in this study
| Strain, plasmid, or primer | Relevant characteristic(s) or nucleotide sequence | Reference or source |
|---|---|---|
| E. coli | ||
| DH5α | supE44 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Laboratory collection |
| SM10λpir | thi thr leu tonA lacY supE recA::RP4-2-Tc::Muλpir, OriT of RP4 Kmr | Laboratory collection |
| V. vulnificus | ||
| ATCC 29307 | Clinical isolate | 7 |
| AR | ATCC 29307, Rifr | Park et al., unpublished |
| KPR101 | AR, ΔrpoS | Park et al., unpublished |
| MO6-24/O | Clinical isolate | 19 |
| MR | MO6-24/O, Rifr | This study |
| HLM101 | MO6-24/O, Δfur | This study |
| Plasmids | ||
| pRK415 | Broad-host-range plasmid | 8 |
| pHK0011 | pRK415, a promoterless luxAB, Tcr | 3 |
| PHL01 | pHK0011, fur::luxAB | This study |
| PHL02 | pHK0011, hupA::luxAB | This study |
| pBluescript SK(+) | pUC19 | New England Biolabs |
| pSKfurup | pBluescriptSK(+), 0.4-kb fur upstream DNA | This study |
| PSKfurup/down | pBluescriptSK(+), 0.9-kb Δfur | This study |
| pDM4 | oriR6K Mob RP4 Cmr | 15 |
| pDMΔfur | pDM4, 0.9 kb Δfur | This study |
| Used for construction of HLM101 | ||
| Primers | ||
| furp-F | 5′-CCCCGGTACCACTCCCGCCATACTGAG-3′ | This study |
| furp-R | 5′-ACCAGGATCCTTTAGCCGTTGGTTATTGTCT-3′ | This study |
| furup-F | 5′-TAAAGAGCTCATACTCCCGCCATACTGA G-3′ | This study |
| furup-R | 5′-ACCAGGATCCTTTAGCGCTTGGTTATTGTCT-3′ | This study |
| furdown-F | 5′-CATGGGATCCTAGTTTGTCTCGACTGCGG-3′ | This study |
| furdown-R | 5′-AAGCGGTACCTGAAAAAGGCTATCAGTTTGA-3′ | This study |
| hupAp-F | 5′-CGCGGGTACCAAGGTTATGGGGCTTTGCA-3′ | This study |
| hupAp-R | 5′-GGGCTCTAGAAGGAACGTTCTGTTGTACAT-3′ | This study |