Graphic
depiction of three operons represented on the microarray. Numbers above
genes indicate wild-type-to-mutant expression ratios in
stationary-phase cells. Arrows indicate transcriptional start sites.
(A) The four genes of the rsbV operon are
transcribed from a σB-dependent promoter
(7). Transcription can
also occur from a σA-dependent promoter further
upstream, which also transcribes the four upstream genes rsbR,
rsbS, rsbT, and rsbU. (B) The four
genes of the opuC operon are transcribed from a
σB-dependent promoter
(22). (C)
Transcription upstream of inlA can be initiated from one of
four promoters (40), one
of which is σB dependent (as confirmed by RACE).
Cotranscription of inlB can occur from at least one of the
inlA promoters
(40). Transcription of
inlB can also occur independently from promoters directly
upstream of inlB (i.e., not including inlA),
including one promoter that does not have a σB
consensus sequence (40)
and potentially from one with a visually predicted
σB-dependent
promoter.