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Brief proteolytic digestion of peripheral G proteins did not affect shear stress-stimulated GTPase activity in reconstituted G proteins. Proteolytic digestion was assessed by GTP[γ-S] binding efficiency (Inset). Integrity of inner leaflet G proteins in trypsinized vesicles was monitored by disrupting with Triton X-100, to make G proteins available for GTP[γ-S] binding.
