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SDS-PAGE of purified E. coli PGPase. Lane 1, crude extract of noninduced BL21(DE3) cells bearing pGEX-Gph; lane 2, crude extract of BL21(DE3) cells bearing pGEX-Gph and induced with IPTG; lane 3, fraction eluted from a glutathione-Sepharose 4B column after digestion with factor Xa; lane 4, fraction eluted from DEAE-Sepharose chromatography; lane M, prestained molecular mass markers (Gibco BRL). Proteins in the gel were visualized by Coomassie brilliant blue staining.
