FIG. 5.

Analysis of expression of the glcD-lacZ transcriptional fusion under oxidative stress. Bleomycin at 5 μg/ml (A) or H₂O₂ at 2 mM (B) was added to aerobic cultures of strain MC4100 (white bars) or JA210 (grey bars) bearing φ(glcD-lacZ). β-Galactosidase activity was measured before and 60 min after the addition of the treatments. Values are means and standard deviations for at least six independent experiments performed in triplicate. A statistically significant difference (P < 0.05) with respect to the nontreated culture is indicated by an asterisk.