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Tyr-705-phosphorylated Stat3α and Stat3β. Stat3 wild-type and Y705F mutant proteins were prepared from Sf9 cells in the presence or absence of JAK-expressing baculovirus. Purified Stat3 proteins were subjected to SDS/PAGE for immunoblot analysis either with anti-Stat3 antibody or with anti-phosphotyrosine antibody.
