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DNA binding activities of Stat3 proteins purified from JAK-containing Sf9 cells. EMSA was performed with two different amounts of each indicated Stat3 protein. (Left) Proteins at 0.1 (right lane of each pair) and 0.2 μg were present. (Right) Amounts of proteins were as follows: Stat3α and 3αY705F, 0.5 (left lane of each pair) and 1 μg, respectively; and Stat3β and 3βY705F, 1 and 2 ng, respectively.
