Table 2.

List of the identified eh1-like motifs in eight subclasses of invertebrate Fox proteins.

Subclass	Protein	Motifsa	Homology to eh1 motifb	Positionc	Protein length	Species	Accession number
A
	FoxA	FAIKNIIA	62.5%	243–250	321	H. vulgaris	AAO92606
	FoxA	FAIKNIIA	62.5%	215–222	286	N. vectensis	42374841
	FoxA	FSIDRIMH	50%	412–419	485	D. japonica	9309317
	FoxA	FSITRLLP	75%	302–309	350	H. armigera	57791692
	FoxA	FSITNLMS	62.5%	375–382	435	P. vulgata	22859616
	FoxA	FSITRLLP	62.5%	300–307	349	B. mori	112983681
	FoxA	FSITRLLP	62.5%	372–379	435	A. aegypti	108881332
	FoxA	FSITRLLP	62.5%	374–381	437	A. gambiae	55233684
	FoxA	FSINRLLP	62.5%	452–459	510	D. melangaster	7301684
	FoxA	FSINRLLP	62.5%	370–377	431	T. castaneum	86515352
	FoxA	FSITRLLP	75%	460–467	570	A. mellifera	110759792
	FoxA	FSINSIIP	62.5%	377–384	440	S. purpuratus	91983614
	FoxA5	FSISSLMN	62.5%	452–459	587	C. intestinalis	AAB61227
	FoxA5	FSISNLMS	87.5%	342–349	403	B. floridae	CAA65368
	FoxA5	FSISSLMN	62.5%	441–448	567	M. oculata	AAB69278
B
	FoxB	FAIENLIG	62.5%	151–158	262	N. vectensis	ABA03229
	FoxB	FSIESILS	75%	229–236	237	C. elegans	AAA28104.1
	FoxB	FTIESLIT	75%	222–229	372	D. melangaster	17977684
	FoxB	FTIESLIT	75%	172–179	241	T. castaneum	91082601
	FoxB	FTIESLIT	75%	189–196	198	A. gambiae	EAA07672
	FoxB	FTIENIIA	75%	313–320	365	A. mellifera	110759134
	FoxB	FTIENIIS	87.5%	187–194	360	S. purpuratus	NP999797
	FoxB	FSIENIIS	87.5%	305–312	475	C. intestinalis	CAD58964
	FoxB	FNIENIIA	62.5%	181–188	289	B. floridae	CAD44627
C
	FoxC	FTVDSLMN	50%	260–267	508	D. melangaster	17975538
	FoxC	FTVDSLMN	50%	266–273	496	A. gambiae	EAA11069
	FoxC	FTVDSLMN	50%	251–258	412	A. aegypti	108876322
	FoxC	FSVDALMN	50%	304–311	495	A. mellifera	110758357
	FoxC	YTVDSLMA	50%	258–265	479	S. purpuratus	72007114
	FoxC	FSVDNIMT	75%	233–300	497	B. floridae	57337372
D
	FoxD	FMISNLLK	75%	434–441	444	S. domuncula	CAE51209
	FoxD	FSMESILS	62.5%	3–10	333	C. elegans	17536629
	FoxD	FSISHIIS	87.5%	393–400	455	D. japonica	BAC10918
	FoxD	FRIETLIG	50%	435–442	456	D. melangaster	17647421
	FoxD	FSIENLIG	75%	491–498	504	A. aegypti	10886922
	FoxD	FSIDALIG	62.5%	313–320	354	A. mellifera	110759337
	FoxD	FTIDSLLN	62.5%	308–315	401	S. purpuratus	115953031
	FoxD	FSIESLIG	62.5%	377–384	506	C. savignyi	BAB68347
	FoxD	FSIENIIG	75%	311–318	402	B. floridae	AF512537
E
	FoxE	FSIENIIG	75%	207–214	393	C. intestinalis	BAC57420
	FoxE4	FSIDNIIA	75%	227–234	381	B. floridae	18653452
G
	FoxG	FSIENILK	75%	12–19	318	M. leidyi	AAN17798
	FoxG	FSIRQMLD	50%	16–23	260	D. japonica	BAC10917
	FoxG	FSILDLCP	37.5%	4–11	270	C. elegans	17569837
	FoxG	FSINSILP	50%	18–25	424	A. gambiae	EAA43390
	FoxG	FGMDRLLG	37.5%	284–291	424	A. gambiae	EAA43390
	FoxG	FSISSILP	75%	156–163	444	T. castaneum	91080905
	FoxG	FNMERLLA	37.5%	381–388	444	T. castaneum	91080905
	FoxG1	FSIRSILP	62.5%	51–58	451	A. mellifera	110756018
	FoxG1	FSMERLLQ	37.5%	328–335	451	A. mellifera	110756018
	FoxG1	FSIDAILA	62.5%	12–19	322	D. melangaster	CAA46890
	FoxG2	FSIDAILP	62.5%	62–69	445	D. melangaster	CAA46891.1
	FoxG	FSVESMLS	62.5%	34–41	507	S. purpuratus	72179617
	FoxG	FSVERLLS	75%	396–403	507	S. purpuratus	72179617
	FoxG1	FSIRRMLS	62.5%	20–27	402	B. floridae	AF067203
	FoxG1	FSVERLLS	75%	286–293	402	B. floridae	AF067203
L
	FoxL1	FTIDNIIG	75%	356–363	365	D. melangaster	Q02360
	FoxL1	FSIDNILA	75%	299–306	521	S. purpuratus	72009133
Q
	FoxQ1	FSIDSILG	62.5%	251–258	408	S. purpuratus	82706210
	FoxQ1	FSIESILS	75%	268–275	385	C. intestinalis	70569660
	FoxQ1	FSIDAILS	75%	226–233	324	B. floridae	CAH55831
	FoxQ2b	FDVESLLR	50%	282–289	380	C. hemisphaerica	108796163
	FoxQ2a	FSIENILG	75%	325–332	387	C. hemisphaerica	108796161
	FoxQ2	FTIEAILE	62.5%	221–228	230	C. elegans	17505695
	FoxQ2	FDVASLLA	50%	348–355	599	D. melangaster	66571262
	FoxQ2	FDVASLLA	50%	233–240	299	T. castaneum	91076112
	FoxQ2	FDVESLLR	50%	232–239	307	A. gambiae	XP566358
	FoxQ2	FSIENLAQ	62.5%	4–11	329	S. purpuratus	ABB89473
	FoxQ2	FSIDRLVG	62.5%	4–11	271	B. floridae	AY163864
Orphans
	Fox1	FRIEFLLK	50%	276–283	285	N. vectensis	ABA03228
	Fox1	FSISKLIL	75%	211–218	218	S. domuncula	CAE51213

^a The highly conserved core of the eh1-like motifs are indicated in bold.

^b The percent similarity between the identified Fox eh1-like motifs and the eh1 motif (FSISNILS) of the Drosophila engrailed homeodomain protein [7].

^c The location of the motifs within the amino acid sequence of the individual Fox proteins.