Table 3.

List of the identified eh1-like motifs in ten subclasses of chordate Fox proteins.

Subclass	Protein	Motifa	Homology to eh1 motifb	Positionc	Protein length	Species	Accession number
A
	FoxA1	FSINNLMS	75%	359–366	427	D. rerio	AAH65668
	FoxA1a	FSINNLMS	75%	356–363	428	X. laevis	AAN76331
	FoxA1b	FSINNLMS	75%	355–362	427	X. laevis	AAA17050
	FoxA1	FSINNLMS	75%	394–401	466	R. norvegicus	6981034
	FoxA1	FSINNLMS	75%	396–403	468	M. musculus	P35582
	FoxA1	FSINNLMS	75%	400–407	472	H. sapiens	24497501
	FoxA2	FSINNLMS	75%	342–349	409	D. rerio	18858687
	FoxA2a	FSINNLMS	75%	351–358	434	X. laevis	45361699
	FoxA2	FSINNLMS	75%	354–361	438	G. gallus	NP990101
	FoxA2	FSINNLMS	75%	377–384	459	M. musculus	6753898
	FoxA2	FSINNLMS	75%	376–383	458	R. norvegicus	NP036875
	FoxA2	FSINNLMS	75%	376–383	457	H. sapiens	24497504
	FoxA3	FSITNLMS	87.5%	376–383	441	D. rerio	18858689
	FoxA3	FSITNLMS	87.5%	259–266	324	S. salar	AAC16333
	FoxA3	FSINNLMS	75%	307–314	353	M. musculus	22477526
	FoxA3	FSINNLMS	75%	394–401	466	R. norvegicus	CAA39418.1
	FoxA3	FSINNLMS	75%	304–311	350	H. sapiens	24497506
	FoxA4	FSITNLMS	87.5%	345–352	417	A. mexicanum	AAC60128
	FoxA4a	FSITQLMS	75%	328–335	399	X. laevis	CAA46290
	FoxA4b	FSITQLMS	75%	328–335	400	X. laevis	AAB22027
	FoxA5	FSISSLMN	62.5%	452–459	587	C. intestinalis	AAB61227
	FoxA5	FSISNLMS	87.5%	342–349	403	B. floridae	CAA65368
	FoxA5	FSISSLMN	62.5%	441–448	567	M. oculata	AAB69278
B
	FoxB	FSIENIIS	87.5%	305–312	475	C. intestinalis	CAD58964
	FoxB	FNIENIIA	62.5%	181–188	289	B. floridae	CAD44627
	FoxB1	FAIENIIA	62.5%	164–171	297	D. rerio	AAH56754
	FoxB1	FAIESIIA	62.5%	171–178	289	T. nigroviridis	47209343
	FoxB1	FAIENIIA	62.5%	167–174	319	X. laevis	AAC62623
	FoxB1a	FAIENIIA	62.5%	170–177	325	M. musculus	Q64732
	FoxB1b	FAIENIIA	62.5%	169–176	324	M. musculus	X92592
	FoxB1	FAIENIIA	62.5%	170–178	324	H. sapiens	Q99853
	FoxB2	FAIENIIG	62.5%	176–183	317	X. laevis	CAD31848
	FoxB2	FAIENIIG	62.5%	267–274	428	M. musculus	NP032049
	FoxB2	FAIENIIG	62.5%	266–273	425	R. norvegicus	109459945
	FoxB2	FAIENIIG	62.5%	270–277	432	H. sapiens	61966923
C
	FoxC	FSVDNIMT	75%	233–300	497	B. floridae	57337372
	FoxC1.1	FSVDNIMT	62.5%	277–284	476	D. rerio	AF219949
	FoxC1.2	FSMDTIMT	75%	254–261	433	D. rerio	AF219950
	FoxC1	FSMDTIMT	75%	275–282	470	T. nigroviridis	47220394
	FoxC1	FSVDNIMT	75%	298–305	495	X. laevis	80478512
	FoxC1	FSVDNIMT	75%	275–282	528	G. gallus	CAA76851
	FoxC1	FSVDNIMT	75%	308–315	553	M. musculus	AAH52011
	FoxC1	FSVDNIMT	75%	307–314	502	B. taurus	76639995
	FoxC1a	FSVDNIMT	75%	308–315	553	H. sapiens	Q12948
	FoxC1b	FSVDNIMT	75%	308–315	553	H. sapiens	AAC72915
	FoxC2	FSVENIMT	75%	258–265	463	X. laevis	47497986
	FoxC2	FSVENIMT	75%	244–251	445	G. gallus	AAC60065
	FoxC2	FSVETIMT	75%	269–276	494	M. musculus	Q61850
	FoxC2	FSVENIMT	75%	270–277	501	H. sapiens	Q99958
D
	FoxD	FSIESLIG	62.5%	377–384	506	C. savignyi	BAB68347
	FoxD	FSIENIIG	75%	311–318	402	B. floridae	AF512537
	FoxD1	FSIDNIIG	75%	295–302	363	D. rerio	AAH75922
	FoxD1.1	FSIDSIIG	62.5%	277–284	343	D. rerio	45501117
	FoxD1	FSIESIIG	62.5%	294–301	345	X. laevis	3892202
	FoxD1	FSIESIIG	62.5%	377–384	440	G. gallus	AAB08467
	FoxD1	FSIESLIG	62.5%	364–371	455	R. norvegicus	XP001057782
	FoxD1	FSIESLIG	62.5%	365–372	456	M. musculus	AAC42042
	FoxD1	FSIESIIG	62.5%	362–369	465	H. sapiens	Q16676
	FoxD2	FSIDNIIG	75%	276–283	346	X. laevis	CAC69867
	FoxD2	FSIDNIIG	75%	365–372	443	G. gallus	AAC60064
	FoxD2	FSIDHIMG	62.5%	409–416	492	M. musculus	NP032619
	FoxD2	FSIDHIMG	62.5%	412–419	495	H. sapiens	55956928
	FoxD3	FSIENIIG	75%	297–304	371	D. rerio	AAC06366
	FoxD3a	FSIENIIG	75%	297–304	371	X. laevis	CAC12963
	FoxD3b	FSIENIIG	75%	297–304	371	X. laevis	CAC12895
	FoxD3	FSIENIIG	75%	319–326	394	G. gallus	AAC60066
	FoxD3	FSIENIIG	75%	366–373	469	M. musculus	NM010425
	FoxD3	FSIENIIG	75%	378–385	478	H. sapiens	NP036315
	FoxD4	FSIESIMQ	62.5%	324–331	408	H. sapiens	18959276
	FoxD4	FTIESIMQ	62.5%	320–327	444	M. musculus	6679841
	FoxD5	FSIDSIMA	62.5%	254–261	321	D. rerio	NP571345
	FoxD5a	FSIENIMR	62.5%	285–292	352	X. laevis	AAD47811
	FoxD5b	FSIENIMK	62.5%	285–292	353	X. laevis	CAB44729
	FoxD5c	FSIENIMG	62.5%	281–288	342	X. laevis	CAB44730
E
	FoxE	FSIENIIG	75%	207–214	393	C. intestinalis	BAC57420
	FoxE1	FRINSLIG	62.5%	202–209	354	D. rerio	XP696065
	FoxE1	FRINNLIG	62.5%	206–213	363	T. nigroviridis	47214250
	FoxE1	FSINTLIG	62.5%	231–238	379	X. laevis	46198238
	FoxE3	FSIDNIIS	87.5%	269–276	422	D. rerio	118918391
	FoxE3	FSIDSLIN	62.5%	215–222	365	X. laevis	6642989
	FoxE3	FSIDSLIS	62.5%	239–246	383	G. galus	118094619
	FoxE3	FRLDSLLG	50%	195–202	288	M. musculus	7657098
	FoxE3	FSVDSLVP	50%	179–186	385	C. familiaris	73977761
	FoxE3	FSVDSLVN	50%	217–224	319	H. sapiens	CAI14973
	FoxE3	FRLDSLLG	50%	193–200	286	R. norvegicus	XP233428
	FoxE4	FSIDNIIA	75%	227–234	381	B. floridae	18653452
G
	FoxG1	FSIRRMLS	62.5%	20–27	402	B. floridae	AF067203
	FoxG1	FSVERLLS	75%	286–293	402	B. floridae	AF067203
	FoxG1	FSINSLVP	62.5%	18–25	420	D. rerio	18858707
	FoxG1	FSINSLMP	62.5%	18–25	436	X. laevis	AAC79501
	FoxG1	FSINSLVP	62.5%	18–25	451	G. gallus	U47275
	FoxG1	FSINSLVP	62.5%	18–25	481	M. musculus	AAB42158
	FoxG1	FSINSLVP	62.5%	18–25	480	R. norvegicus	6978845
	FoxG1a	FSINSLVP	62.5%	18–25	469	H. sapiens	CAA55038
	FoxG1b	FSINSLVP	62.5%	18–25	477	H. sapiens	X74142
H
	FoxH1	FAIDSLLH	50%	250–257	472	D. rerio	18858709
	FoxH1	FAIDSLLH	50%	278–285	285	T. nigroviridis	47223489
	FoxH1	FMIDSLLH	50%	271–278	518	X. laevis	P70056
	FoxH1	FSIKSLLG	62.5%	198–205	401	R. norvegicus	XP235454
	FoxH1	FSIKSLLG	62.5%	167–174	310	B. taurus	CAD58794
	FoxH1	FSIKSLLG	62.5%	198–205	401	M. musculus	6679845
	FoxH1	FSIKSLLG	62.5%	194–201	612	H. sapiens	41107639
I
	FoxI1	FSVNNLIY	75%	405–412	419	D. rerio	AAO63568
	FoxI1c	FSVNSLIY	62.5%	367–374	381	X. laevis	CAD31849
	FoxI1c	FTVNSLIY	62.5%	345–352	359	G. gallus	50747424
	FoxI2	FSVNSLIY	62.5%	369–376	383	D. rerio	AAP92808
Q
	FoxQ1	FSIESILS	75%	268–275	385	C. intestinalis	70569660
	FoxQ1	FSIDAILS	75%	226–233	324	B. floridae	CAH55831
	FoxQ1	FAIDSILS	62.5%	177–184	383	D. rerio	AAH67139
	FoxQ1	FRIDSLLS	62.5%	276–283	383	D. rerio	AAH67139
	FoxQ1	FTIDSILS	75%	196–203	272	T. nigroviridis	47220396
	FoxQ1	FAIDSILS	62.5%	224–231	381	X. laevis	76152394
	FoxQ1	FAIDSILS	62.5%	268–275	400	M. musculus	31560693
	FoxQ1	FAIDSILS	62.5%	252–259	439	R. norvegicus	12408312
	FoxQ1	FAIDSILR	50%	270–277	402	H. sapiens	8489093
	FoxQ2	FTIDYLLY	62.5%	17–24	244	D. rerio	XP694156
	FoxQ2	FTIDYLLF	62.5%	20–27	210	T. nigroviridis	47209212
	FoxQ2	FSIDRLVG	62.5%	4–110	271	B. floridae	AY163864
L
	FoxL1	FSIDSILS	75%	284–291	363	D. rerio	41055835
	FoxL1	FSIDSILA	62.5%	255–262	336	M. musculus	NP032050
	FoxL1	FSIDSILA	62.5%	259–266	389	R. norvegicus	109508994
	FoxL1	FSIDSILA	62.5%	262–269	346	B. taurus	61823329
	FoxL1	FSIDSILA	62.5%	272–279	356	C. familiaris	73956953
	FoxL1	FSIDSILA	62.5%	261–268	245	H. sapiens	22779860

^a The highly conserved core of the eh1-like motifs are indicated in bold.

^b The percent similarity between the identified Fox eh1-like motifs and the eh1 motif (FSISNILS) of the Drosophila engrailed homeodomain protein [7].

^cThe location of the motifs within the amino acid sequence of the individual Fox proteins.