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Real time PCR for prolactin mRNA in total RNA from normal and BDL female cholangiocytes. We demonstrated that: (i) female normal cholangiocytes express prolactin mRNA at low levels; and (ii) following BDL, prolactin mRNA markedly increased in female cholangiocytes. Data are mean ± SEM of 3 experiments. *p < 0.05 vs. relative expression of prolactin receptor of normal female cholangiocytes.
