TABLE 4.
Characteristics of gene products deduced from ORFs identified within the 35-kb genomic region of M. gryphiswaldense MSR-1
| ORFa | Size of gene product (amino acids) | Best BLAST hitb (accession no.) | E value | Homologues in magnetotactic bacteriac (accession no.) | E value | Proposed function of BLAST homolog | |
|---|---|---|---|---|---|---|---|
| ORF 1 | 230 | M. mag. (ZP_00055313.1) | 4e-05 | Unknown | |||
| MC-1 (ZP_00047740.1) | 0.003 | ||||||
| ORF 2 | 347 | r03081 Sinorhizobium meliloti (AL591792) | 1e-05 | M. mag. (ZP_00053421.1) | 8e-77 | Unknown transmembrane | |
| ORF 3 | 107 | — | M. mag. (ZP_00053420.1) | 6e-43 | Unknown | ||
| MC-1 (ZP_00042693.1) | 2e-07 | ||||||
| ORF 4 | 136 | — | mms6 AMB-1 (BAC65162.1) | 7e-11 | Iron binding | ||
| M. mag. (ZP_00053419.1) | 7e-11 | ||||||
| mamG | 84 | — | M. mag. (ZP_00050810.1) | 0.37 | Unknown | ||
| mamF | 111 | abf1 Kluyveromyces marxianus (Z19865) | 0.003 | M. mag. (ZP_00053416.1) | 9e-41 | Transcription regulation | |
| MC-1 (ZP_00042693.1) | 3e-09 | ||||||
| mamD | 314 | — | M. mag. (ZP_00053415.1) | 4e-88 | Unknown | ||
| mms7 AMB-1 (BAC65161.1) | 1e-87 | ||||||
| MC-1 (ZP_00043592.1) | 2e-11 | ||||||
| mamC | 125 | — | M. mag. (ZP_00053414.1) | 4e-16 | Unknown | ||
| mms13 AMB-1 (BAC65160.1) | 4e-16 | ||||||
| MC-1 (ZP_00042848.1) | 2e-04 | ||||||
| ORF 9 | 278 | idiA Synechococcus sp. (Z48754) | 9e-75 | — | Ferric iron transport | ||
| ORF 10 | 214 | TRm2011 Sinorhizobium meliloti (NP_435256.1) | 2e-30 | MC-1 (ZP_00049672.1) | 9e-41 | Transposase | |
| M. mag. (ZP_00052583.1) | 2e-37 | ||||||
| ORF 11 | 436 | istA Agrobacterium tumefaciens (AE009415) | 1e-90 | MC-1 (ZP_00042589.1) | 5e-25 | IS21 family transposase | |
| ORF 12 | 525 | PA1673 Pseudomonas aeruginosa (AE004595) | 8e-11 | M. mag. (ZP_00054420.1) | 2e-17 | Hemerythrin-like | |
| MC-1 (ZP_00043360.1) | 4e-07 | ||||||
| ORF 13 | 149 | PA1673 Pseudomonas aeruginosa (AE004595) | 3e-07 | M. mag. (ZP_00052998.1) | 1e-35 | Hemerythrin-like | |
| MC-1 (ZP_00044836.1) | 9e-11 | ||||||
| ORF 14 | 317 | Acetobacter xylinus (S35004) | 3e-46 | M. mag. (ZP_00052299.1) | 3e-17 | Transposase_11 IS1031C | |
| MC-1 (ZP_00048870.1) | 2e-09 | ||||||
| ORF 15 | 76 | MSR8681 Rhizobium loti (Q98AK2) | 1e-20 | M. mag. (ZP_00052258.1) | 2e-07 | Unknown | |
| ORF 16 | 415 | MLL1146 Rhizobium loti (Q98L77) | 3e-45 | M. mag. (ZP_00053281.1) | 0 | Unknown | |
| mamH | 431 | CT1475 Chlorobium tepidum (Q8KCE7) | 8e-35 | M. mag. (ZP_00053280.1) | e-170 | Sugar (and other) transporter | |
| MC-1 (ZP_00043594.1) | 5e-77 | ||||||
| mamI | 87 | MC-1 (ZP_00042845.1) | 0.002 | Unknown | |||
| mamE | 772 | MLL5022 Rhizobium loti (Q98CS8) | 9e-35 | mamE M. mag. (ZP_00054403.1) | 0 | Serine protease | |
| MC-1 (ZP_00042846.1) | 5e-55 | ||||||
| mamJ | 466 | SP1772 Streptococcus pneumoniae (E95206) | 0.001 | mamJ M. mag. (ZP_00054404.1) | 2e-32 | Cell wall surface anchor family | |
| mamK | 347 | mreB Methanopyrus kandleri (QSTYX3) | 2e-17 | mamK M. mag. (ZP_00054405.1) | e-159 | Actin-like | |
| MC-1 (ZP_00042854.1) | 6e-84 | ||||||
| mamL | 123 | — | mamL M. mag. (ZP_00054405.1) | 3e-07 | Unknown | ||
| MC-1 (ZP_00042854.1) | 0.048 | ||||||
| mamM | 318 | BH1238 Bacillus halodurans (F83804) | 1e-33 | mamM M. mag. (ZP_00054406.1) | e-171 | Cation efflux | |
| mamM MC-1 (ZP_00043611.1) | 4e-77 | ||||||
| mamN (ORF 1) | 437 | TM0934 Thermotoga maritima (AE001757) | 6e-30 | mamN M. mag. (ZP_00054407.1) | 0 | Transporter | |
| mamO (ORF 2) | 632 | CC1282 Caulobacter crescentus (C87408) | 6e-15 | mamO M. mag. (ZP_00054408.1) | 0 | Serine protease | |
| mamO MC-1 (ZP_00043610.1) | 7e-77 | ||||||
| mamP (ORF 3) | 270 | OrfE0 Rhodobacter capsulatus (CAA72164) | 0.042 | mamP M. mag. (ZP_0054409.1) | e-106 | Serine protease | |
| mamP MC-1 (ZP_00043609.1) | 1e-32 | ||||||
| mamA (ORF 4) | 217 | MM2348 Methanosarcina mazei (AE013478) | 3e-15 | mamA M. mag. (BAA11643.2) | e-111 | TPR protein | |
| mamA MC-1 (ZP_00043608.1) | 2e-35 | ||||||
| mamQ (ORF 5) | 272 | lemA Themotoga maritima (AE001759) | 3e-16 | mamQ M. mag. (ZP_00054411.1) | e-109 | Unknown | |
| mamQ MC-1 (ZP_0043606.1) | 1e-35 | ||||||
| mamR (ORF 6) | 72 | mamR M. mag. (ZP_00054412.1) | 4e-29 | ||||
| mamB (ORF 7) | 297 | ydfM Bacillus subtilis (C69781) | 1e-37 | mamB M. mag. (ZP_00054413.1) | e-156 | Cation efflux | |
| mamB MC-1 (ZP_00043605.1) | 7e-76 | ||||||
| mamS (ORF 8) | 180 | — | mamS M. magn. (ZP_00054414.1) | 1e-58 | Unknown | ||
| — | mamS MC-1 (ZP_00043604.1) | 3e-11 | |||||
| mamT (ORF 9) | 174 | mamT M. mag. (ZP_00054415.1) | 2e-81 | Unknown (heme binding) | |||
| mamT MC-1 (ZP_00043609.1) | 5e-04 | ||||||
| mamU (ORF 10) | 297 | MLL9677 Rhizobium loti (AP003017) | 6e-22 | mamU M. mag. (ZP_00054416.1) | e-115 | Unknown | |
| ORF 34 | 411 | SP1776 Streptococcus pneumoniae (E95206) | 7e-05 | — | Cell wall surface anchor family | ||
| ORF 35 | 254 | y4hP Rhizobium sp. (T10850) | 3e-76 | M. mag. (ZP_00052071.1) | 2e-78 | Transposase_25 IS66 | |
| MC-1 (ZP_000442447.1) | 8e-37 | ||||||
| ORF 36 | 104 | Agrobacterium tumefaciens (Q52599) | 4e-16 | MC-1 (ZP_00049269.1) | 2e-07 | Transposase_25 IS66 | |
| ORF 37 | 341 | y4hP Rhizobium sp. (T10850) | 1e-108 | M. mag. (ZP_00052071.1) | 2e-76 | Transposase_25 IS66 | |
| MC-1 (ZP_00047840.1) | 3e-41 |
a
Genes which encode known magnetosome-associated proteins are in bold. Numbers in parentheses refer to the previously described ORF in M. gryphiswaldense (16).
b
Only hits in organisms other than those shown in the homologues column are shown.
c
MC-1, Magnetococcus sp. strain MC-1; AMB-1, Magnetospirillum sp. strain AMB-1; M. mag., Magnetospirillum magnetotacticum strain MS-1.