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. 2007 Aug 15;2(8):e729. doi: 10.1371/journal.pone.0000729

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Normal or Tangier human fibroblasts, (B) EBV-transformed normal or Tangier B lymphocytes, or (C and D) thymocytes from wildtype or ABCA1^−/ ⁻ mice were treated with Ca²⁺ and Ca²⁺ ionophore, and cellular fluorescence in the presence of fluorescent annexin V measured continuously over time at room temperature. Normal/wildtype, filled circles; ABCA1-deficient, open circles. A–C, median fluorescence of cells; D, fraction of cells stained by fluorescent annexin.