Temperature-sensitive growth of relA mutants. A set of isogenic strains carrying three common relA mutant alleles were constructed. These strains were compared for growth on nutrient agar at temperatures ranging from 30 to 43°C. Growth is expressed as plating efficiency (plate counts at the high temperature divided by plate counts at 30°C). a, strain VC6132 (wild type); b, strain VC6129 (ΔrelA251::kan); c, strain VC6130 (ΔrelA251::kan ΔspoT207::cat); d, strain VC6133 (relA2); e, strain VC6141 (relA1).