TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotype or description | Source (reference) |
|---|---|---|
| Bacteria | ||
| CF1693 | ΔrelA251::kan ΔspoT207::cat | M. Cashel |
| CF1736 | pyrE+spoT202 | M. Cashel |
| CF1738 | pyrE+spoT203 | M. Cashel |
| CF1740 | pyrE+spoT204 | M. Cashel |
| CF1964 | btuB::Tn10 rpoB7 | M. Cashel |
| CF1969 | btuB::Tn10 rpoB3443 | M. Cashel |
| CF1970 | btuB::Tn10 rpoB3449 | M. Cashel |
| CF1971 | btuB::Tn10 rpoB114 | M. Cashel |
| CF1972 | btuB::Tn10 rpoB337 | M. Cashel |
| CF4268 | btuB::Tn10 rpoB3445 | M. Cashel |
| CF4297 | btuB::Tn10 rpoB3401 | M. Cashel |
| CF5034 | pyrE60 zib-563::Tn10 | M. Cashel |
| CP79 | relA2 | Laboratory collection |
| MC4100 | relA1 | Laboratory collection |
| VC348 | zei-348::Tn5 | Laboratory collection |
| VC6129 | W3110 ΔrelA251::kan (W3110 × CF1693) | This study |
| VC6130 | VC6129 ΔspoT207::cat (VC6129 × CF1693) | This study |
| VC6132 | W3110 zei-348::Tn5 relA+ | This study |
| VC6133 | W3110 zei-348::Tn5 relA2 | This study |
| VC6141 | W3110 zei-348::Tn5 relA1 | This study |
| VC6159 | VC6129 btuB::Tn10 rpoB+ (VC6129 × CF1970) | This study |
| VC6158 | VC6129 btuB::Tn10 rpoB3449 (VC6129 × CF1970) | This study |
| VC6160 | VC6129 btuB::Tn10 rpoB3401 (VC6129 × CF4297) | This study |
| VC6166 | VC6129 btuB::Tn10 rpoB3445 (VC6129 × CF4268) | This study |
| VC6173 | VC6129 btuB::Tn10 rpoB7 (VC6129 × CF1964) | This study |
| VC6191 | VC6129 btuB::Tn10 rpoB114 (VC6129 × CF1971) | This study |
| VC6192 | VC6129 btuB::Tn10 rpoB3443 (VC6129 × CF1969) | This study |
| VC6193 | VC6129 btuB::Tn10 rpoB3370 (VC6129 × CF1972) | This study |
| VC7237 | VC6141 zib-563::Tn10 | This study |
| VC7238 | VC6141 pyrE60 zib-563::Tn10 (VC6141 × CF5034) | This study |
| VC7239 | VC6141 spoT202 (VC6141 × CF1736) | This study |
| VC7240 | VC6141 spoT203 (VC6141 × CF1738) | This study |
| VC7241 | VC6141 spoT204 (VC6141 × CF1740) | This study |
| W3110 | Prototrophic | Laboratory collection |
| Plasmids | ||
| pALS10 | Apr; E. coli relA clone | J. Zyskind (23) |
| pALS13 | Apr; truncated E. coli relA clone | J. Zyskind (23) |
| pDJJ12 | Apr; ColE1 derivative carrying E. coli rpoBC | M. Cashel (11) |
| pDS1 | Cmr; E. coli rpoH in pACYC184 | C. A. Gross |