. Author manuscript; available in PMC: 2007 Aug 3.

Published in final edited form as: Dev Biol. 2007 Mar 3;305(2):564–576. doi: 10.1016/j.ydbio.2007.02.037

Table 1.

Mutation of E2f4 results in an increase in the percentage of nasal respiratory epithelium cells that stain with PAS and Alcian Blue.

	PAS	Alcian Blue
Wild-type and E2f4 +/−	16.3 +/− 6.6	14.4 +/− 4.3
E2f4 −/−	81 +/− 5.1	76 +/− 6.8
n (pairs)	5	4
p value	<0.0001	0.001

For each stain pairs of matched sections from an 18.5 dpc E2f4−/− embryo and a wild-type or E2f4+/− littermate were analyzed. A minimum of 120 epithelial nuclei were counted per section and each cell scored for staining with either PAS or Alcian Blue. The number of pairs of independent embryos analyzed is indicated (n). The mean % of positive cells +/− standard deviation is presented and the data shown to be highly significant in both cases using a paired Student’s t-test.