Table 2.
Quantification of CC10 and SP-C marker expression.
| CC10 | SP-C | |
|---|---|---|
| Wild-type and E2f4 +/− | 64.4 +/− 10.1 | 7.7+/− 2.3 |
| E2f4 −/− | 34.1 +/− 17.1 | 8 +/− 2.8 |
| n (pairs) | 8 | 4 |
| p value | 0.00013 | 0.31 |
For each stain pairs of matched sections from an 18.5 dpc E2f4−/− embryo and a wild-type or E2f4+/− littermate were analyzed. A minimum of 200 bronchiolar (CC10) or 350 lung (SP-C) nuclei were counted per section and each cell scored for strong staining with CC10 or staining with SP-C antibodies. The number of pairs of independent embryos analyzed is indicated (n). The mean % of positive cells +/− standard deviation is presented and the data analyzed using a paired Student’s t-test and the p values indicated.