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. Author manuscript; available in PMC: 2008 May 31.
Published in final edited form as: J Immunol Methods. 2007 Apr 5;323(1):11–23. doi: 10.1016/j.jim.2007.03.006

Figure 4.

Figure 4

Specificity of mAb L317 for the KRN7000:mCD1d complex. Biotinylated L317 (1 nM) was allowed to bind to platebound KRN7000:mCD1d complexes in the presence of increasing concentrations of soluble KRN7000-loaded mCD1d, free KRN7000, iGb3-loaded mCD1d, or KRN7000-loaded human CD1d. Bound L317-biotin was detected with AP-streptavidin. Signal was normalized to mAb binding in the absence of inhibitor (100%), and binding to buffer-coated wells (0%). Similar results were obtained with mAbs L363 and L386.