Figure 7.
Binding of mAb L363 to mCD1d loaded with other α-GalCer lipids. Binding of mAb L363-biotin to ELISA plate wells coated with varying concentrations of mCD1d dimers was detected with AP-streptavidin. mCD1d protein was pre-loaded with KRN7000, α-GalCer C20:2, OCH, iGb3, or solvent vehicle in the presence of 0.05% Triton X-100.