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Intracellular cytokine staining in CD4+ T cells. CD4+ T cells from the lung or bronchial lymph nodes (BLN) from either TCR-HA control mice, SPC-HA/TCR-HA double transgenic mice or SPC-HA mice adoptively transferred with HA-specific CD4+ T cells were analyzed by FACS for the expression of interleukin 2 and interferon γ.
