Figure 5. Spatial Learning Promotes the Death of Newborn Neurons Generated a Few Days before Training.
(A) Latency to find the escape platform. Learning groups were injected with BrdU either 4 or 3 d before initiation of training as indicated by the syringes. D, day.
(B) Newly born cells measured by the number of BrdU-IR cells.
(C and D) Apoptotic cells measured by the number of fractin-IR cells (C) and both pyknotic and karyorrhexic dying cells (D).
(E) Number of BrdU-IR cells exhibiting characteristics of dying cells.
(F) Percentage of BrdU-IR cells expressing the immature neuronal marker Dcx.
(G) Extrapolated number of newborn neurons.
A single asterisk (*) indicates p ≤ 0.05, double asterisks (**) indicate p ≤ 0.01, and triple asterisks (***) indicate p ≤ 0.001 compared to Control groups. Error bars represent the s.e.m.