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. 2007 Jun 5;2007:27683. doi: 10.1155/2007/27683

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Copyright © 2007 Alexandros Basilios Tsoupras et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Dependence of PAF formation on incubation time and protein concentration: (a) time course of PAF production using 0.025, 0.05, and 0.1 mg total protein/mL; (b) CPT activity as a function of protein concentration at a fixed incubation time of 20 minutes. Experiments were performed with microsomal fractions of HMC in the presence of 100 μM AAG and 100 μM CDP-choline. Results are the average of two independent determinations using different enzyme preparations performing duplicate samples.