Table 3.
Effect of Ca2+ cation on PAF-CPT activity of HMC. Results represent the average of two independent experiments using different enzyme preparations performing duplicate samples.
| Ca2+ cation | Concentration in assay (mM) | PAF-CPT activity (%) |
|
| ||
| Exogenous Ca2+ ([EDTA] = 0.5 mM, [Mg2+] = 20 mM) | 0 | 100 |
| Exogenous Ca2+ ([EDTA] = 0, [Mg2+] = 20 mM) | 0 | 67.4 |
| Exogenous Ca2+ ([EDTA] = 0, [Mg2+] = 20 mM) | 0.1 | 14.1 |
| Exogenous Ca2+ ([EDTA] = 0.5 mM, [Mg2+] = 20 mM) | 0.1 | 33.3 |
| Exogenous Ca2+ ([EDTA] = 0, [Mg2+] = 20 mM) | 1 | 1.41 |
| Exogenous Ca2+ ([EDTA] = 0.5 mM, [Mg2+]= 20 mM) | 1 | 3.70 |