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Immunolocalization of nestin, A2B5, and GFAP in S10 neurospheres. In (a), (b), and (c), double immunostaining of nestin/Hoechst, A2B5/Hoechst, and GFAP/Hoechst is shown, respectively. In (d), (e), and (f), the single immunostaining is shown. Bar = 40 μm.
