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. Author manuscript; available in PMC: 2007 Sep 9.
Published in final edited form as: Mol Cell. 2007 Mar 9;25(5):689–702. doi: 10.1016/j.molcel.2007.02.012

Figure 4.

Figure 4

Ime2 phosphates are completely resistant to Cdc14 activity

(A) A model substrate (Fin1) was phosphorylated by Cdk1 or Ime2 and the rate of phosphate release upon incubation with Cdc14, PP2A-Cdc55 or PP2C was determined by TCA precipitation and scintillation counting of soluble phosphate.

(B) APCCdh1 can be reactivated by Cdc14 after phosphorylation by Cdk1 but not Ime2. A reconstituted APC assay was performed using Cdh1 that was first phosphorylated by either Cdk1 or Ime2 and then treated with increasing amounts of Cdc14 (SDS-PAGE and autoradiography of 35S-securin on the left; quantitation on the right. Data are represented as mean +/- SD).

(C) APCCdh1 is controlled by a complex variety of kinases and phosphatases. The ability of different phosphatases to reactivate Cdh1 following Ime2 or Cdk1 phosphorylation was assessed as in panel B.