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Nuclear gems/CBs and cytoplasmic levels of most gemins are greatly reduced in SMN-deficient human skin fibroblasts, but cytoplasmic gemin5 levels remain high. Alexa fluor 546 goat anti-(mouse IgG) (red) was used to detect SMN and gemin 2, 4, 5, 6 and 7 primary mAbs (the same mAbs as in Fig. 2). TRITC donkey anti-(rabbit Ig) was used to detect anti-gemin3 rabbit antibodies. DAPI (blue) was used to counterstain the nuclei.
