Figure 7.

Distribution of gemins and other proteins between cytoplasmic and nuclear fractions of HeLa and Ntera2 cells. HeLa C and Ntera C are cytoplasmic fractions, HeLa N and Ntera N are nuclear fractions. After extraction with cytoplasmic buffer (see Methods), the whole pellet was boiled in SDS sample buffer to obtain the "nuclear" extract. Samples were loaded as serial dilutions (from left to right, 1/4, 1/2 and 1) for more accurate microdensitometry quantitation. γ tubulin and lamin A/C were used as controls for cross-contamination between nuclear and cytoplasmic fractions.