Table 4.
Subset of E. histolytica genes upregulated by exposure to Trichostatin A.
| Probe ID | GenBank ID | Description | Fold-change | FDR |
| 489.m00024_at | XM_643195 | hypothetical protein | 39.96 | 7.60E-16 |
| 14.m00310_at | XM_651236 | hypothetical protein | 39.67 | 2.32E-20 |
| 295.m00030_at | XM_644416 | conserved hypothetical protein | 38.77 | 2.29E-20 |
| 522.m00017_at | XM_643097 | hypothetical protein | 20.45 | 4.53E-15 |
| 418.m00028_at | XM_643537 | 70 kDa heat shock protein, putative | 17.45 | 6.71E-10 |
| 36.m00204_s_at | XM_650008 | hypothetical protein | 14.84 | 1.22E-08 |
| 451.m00037_s_at | XM_643383 | hypothetical protein | 14.68 | 3.96E-04 |
| 556.m00022_x_at | XM_643031 | hypothetical protein | 14.37 | 5.75E-07 |
| 12.m00306_at | XM_651386 | hypothetical protein | 13.25 | 1.64E-05 |
| 376.m00054_s_at | XM_643784 | hypothetical protein | 13.02 | 1.62E-10 |
| 564.m00020_x_at | XM_643013 | hypothetical protein | 12.93 | 8.19E-10 |
| 82.m00164_s_at | XM_648353 | hypothetical protein | 12.88 | 8.00E-12 |
| 28.m00298_s_at | XM_650406 | hypothetical protein | 11.79 | 5.97E-09 |
| 110.m00118_at | XM_647568 | Rho family GTPase | 11.60 | 2.26E-14 |
| 50.m00195_s_at | XM_649449 | hypothetical protein | 11.36 | 3.02E-05 |
| 135.m00094_at | XM_646923 | hypothetical protein | 10.98 | 2.38E-08 |
| 493.m00030_x_at | XM_643175 | hypothetical protein | 10.25 | 1.40E-24 |
| 337.m00049_at | XM_644075 | hypothetical protein | 9.93 | 5.43E-07 |
| 164.m00105_x_at | XM_646368 | hypothetical protein | 9.92 | 4.32E-05 |
| 847.m00011_x_at | XM_642805 | hypothetical protein | 9.63 | 9.76E-05 |
| 205.m00100_s_at | XM_645582 | hypothetical protein | 9.48 | 3.01E-08 |
| 373.m00052_at | XM_643804 | hypothetical protein | 8.71 | 1.14E-06 |
| 395.m00030_x_at | XM_643673 | protein kinase, putative | 8.68 | 6.64E-04 |
| 749.m00013_s_at | XM_642848 | hypothetical protein | 8.61 | 1.85E-03 |
| 227.m00077_at | XM_645230 | hypothetical protein | 8.40 | 1.70E-08 |
| 728.m00012_x_at | XM_642855 | hypothetical protein | 8.37 | 5.83E-06 |
| 460.m00025_x_at | XM_643328 | hypothetical protein | 8.00 | 3.05E-02 |
| 167.m00116_x_at | XM_646306 | hypothetical protein | 7.98 | 3.77E-03 |
| 621.m00019_at | XM_642928 | hypothetical protein | 7.84 | 8.57E-06 |
| 584.m00019_at | XM_642979 | heat shock protein 70, putative | 7.82 | 5.54E-06 |
| 477.m00021_at | XM_643239 | hypothetical protein | 7.60 | 1.27E-03 |
| 123.m00123_x_at | XM_647209 | conserved hypothetical protein | 7.48 | 3.84E-05 |
| 76.m00146_at | XM_648554 | hypothetical protein | 7.34 | 1.75E-04 |
| 89.m00125_s_at | XM_648139 | hypothetical protein | 7.32 | 2.51E-05 |
| 220.m00068_x_at | XM_645332 | hypothetical protein | 7.14 | 2.48E-03 |
| 263.m00053_at | XM_644795 | hypothetical protein | 6.95 | 2.56E-04 |
| 804.m00006_x_at | XM_642821 | hypothetical protein | 6.94 | 2.44E-04 |
| 72.m00179_at | XM_648717 | hypothetical protein | 6.85 | 1.17E-04 |
| 211.m00072_at | XM_645468 | zinc finger protein, putative | 6.80 | 5.04E-03 |
| 102.m00082_at | XM_647784 | hypothetical protein | 6.74 | 6.19E-03 |
| 411.m00025_x_at | XM_643570 | hypothetical protein | 6.68 | 8.26E-03 |
| 162.m00085_at | XM_646412 | predicted protein | 6.61 | 3.81E-06 |
| 157.m00087_x_at | XM_646502 | conserved hypothetical protein | 6.50 | 8.44E-04 |
| 12.m00326_at | XM_651342 | hypothetical protein | 6.42 | 4.10E-04 |
| 52.m00169_x_at | XM_649399 | hypothetical protein | 6.39 | 2.21E-03 |
| 22.m00288_at | XM_650731 | hypothetical protein | 6.28 | 6.49E-04 |
| 71.m00129_at | XM_648728 | hypothetical protein | 6.16 | 5.70E-05 |
| 6.m00428_at | XM_651814 | hypothetical protein | 6.10 | 1.46E-04 |
| 496.m00027_x_at | XM_643167 | hypothetical protein | 5.98 | 9.24E-06 |
| 135.m00113_at | XM_646942 | hypothetical protein | 5.91 | 4.26E-03 |
The mean trophozoite expression value for E. histolytica 200:NIH strain under standard culture conditions, the fold-change in TSA treated parasites, FDR, GenBank ID number, and gene annotations are shown. Genes in bold have been confirmed by RT-PCR. Genes regulated during trophozoite to cyst development are shown in italics. Additional loci represented by crosshybridizing _s_at probe sets are as follows: 36.m00204_s_at: 88.m00180; 451.m00037_s_at: 467.m00031 and 50.m00196; 376.m00054_s_at: 116.m00123; 82.m00164_s_at: 82.m00157; 28.m00298_s_at: 481.m00033 and 90.m00158; 205.m00100_s_at: 105.m00129; 749.m00013_s_at: 142.m00151; 89.m00125_s_at: 357.m00038.