Table 5.
E. histolytica genes downregulated by exposure to Trichostatin A.
| Probe ID | GenBank ID | Description | Fold-change | FDR |
| 111.m00118_at | XM_647540 | leishmaniolysin-related peptidase, putative | -44.44 | 0.00E+00 |
| 233.m00105_at | XM_645153 | hypothetical protein | -29.24 | 0.00E+00 |
| 7.m00429_at | XM_651789 | Beige BEACH domain protein, putative | -21.88 | 8.51E-10 |
| 223.m00071_at | XM_645286 | hypothetical protein | -10.88 | 3.04E-04 |
| 31.m00224_at | XM_650243 | hypothetical protein | -9.43 | 2.76E-03 |
| 1.m00712_at | XM_652408 | hypothetical protein | -8.62 | 1.64E-05 |
| 223.m00075_at | XM_645290 | lipid phosphatase, putative | -6.71 | 1.34E-03 |
| 105.m00133_at | XM_647680 | NADP-dependent alcohol dehydrogenase | -5.81 | 8.19E-05 |
| 223.m00077_x_at | XM_645292 | hypothetical protein | -5.65 | 8.47E-03 |
| 585.m00015_s_at | XM_642978 | conserved hypothetical protein | -5.52 | 1.11E-02 |
| 223.m00069_at | XM_645284 | hypothetical protein | -5.49 | 9.05E-03 |
| 223.m00068_at | XM_645283 | scavenger mRNA decapping enzyme, putative | -5.24 | 2.27E-03 |
| 17.m00307_s_at | XM_651008 | fatty acid elongase, putative | -5.08 | 1.34E-02 |
| 234.m00042_at | XM_645139 | hypothetical protein | -5.00 | 2.34E-02 |
| 66.m00150_at | XM_648887 | high mobility group protein, putative | -4.78 | 3.68E-02 |
| 131.m00139_at | XM_647034 | M-phase inducer phosphatase, putative | -4.72 | 2.36E-02 |
| 249.m00083_at | XM_644976 | hypothetical protein | -4.44 | 3.58E-03 |
| 7.m00436_at | XM_651745 | actobindin homolog, putative | -4.31 | 7.67E-03 |
| 223.m00079_at | XM_645294 | cysteinyl-tRNA synthetase, putative | -4.20 | 1.54E-02 |
| 425.m00057_s_at | XM_643497 | conserved hypothetical protein | -4.10 | 2.14E-02 |
| 223.m00070_at | XM_645285 | protein kinase, putative | -4.08 | 2.28E-02 |
| 4.m00636_at | NA | pseudogene, galactose-specific adhesin light subunit | -4.00 | 4.34E-02 |
| 217.m00081_s_at | XM_645388 | hypothetical protein | -3.91 | 1.64E-02 |
| 92.m00148_s_at | XM_648072 | conserved hypothetical protein | -3.88 | 4.66E-02 |
| 223.m00078_at | XM_645293 | ribonuclease, putative | -3.85 | 3.89E-02 |
| 180.m00114_at | XM_646068 | hypothetical protein | -3.73 | 1.75E-03 |
| 223.m00067_at | XM_645282 | integral membrane protein, putative | -3.73 | 3.58E-03 |
| 223.m00074_at | XM_645289 | hypothetical protein | -3.72 | 1.80E-02 |
| 223.m00076_at | XM_645291 | hypothetical protein | -3.42 | 9.24E-03 |
| 10.m00362_at | XM_651510 | cysteine proteinase, putative | -3.41 | 3.20E-09 |
| 54.m00183_at | XM_649345 | hypothetical protein | -3.33 | 4.69E-02 |
| 242.m00078_s_at | XM_645064 | cysteine protease 1 | -2.89 | 3.47E-03 |
| 77.m00173_at | XM_648536 | hypothetical protein | -2.76 | 2.82E-04 |
| 17.m00351_at | XM_651053 | galactose-inhibitable lectin 35 kda subunit precursor | -2.75 | 2.84E-02 |
| 9.m00419_at | XM_651593 | Fe-hydrogenase, putative | -2.72 | 1.73E-03 |
| 52.m00148_at | XM_649403 | lysozyme, putative | -2.61 | 4.36E-02 |
| 154.m00120_at | XM_646541 | glucosidase II alpha subunit, putative | -2.57 | 3.53E-02 |
| 9.m00416_at | XM_651590 | hypothetical protein | -2.49 | 4.28E-02 |
| 47.m00182_at | XM_649559 | fatty acid elongase, putative | -2.26 | 3.57E-02 |
| 22.m00269_s_at | XM_650745 | hypothetical protein | -2.19 | 2.76E-02 |
| 77.m00178_s_at | XM_648541 | surface antigen ariel1-related | -2.08 | 3.15E-02 |
The mean trophozoite expression value for E. histolytica 200:NIH strain under standard culture conditions, the fold-change in TSA treated parasites, FDR, GenBank ID number, and gene annotations are shown. Genes in bold have been confirmed by RT-PCR. Genes regulated during trophozoite to cyst development are shown shaded in italics. Additional loci represented by crosshybridizing _s_at probe sets are as follows: 17.m00307_s_at: 17.m00304; 425.m00057_s_at: 10.m00394; 217.m00081_s_at: 20.m00277; 92.m00148_s_at: 250.m00108 and 284.m00087; 242.m00078_s_at: 79.m00156; 77.m00178_s_at: 19.m00343 and 533.m00017.