Fig. 3.

Cholera toxin induces proliferation inhibition but does not induce cell death of glioma cells. Cells were incubated with 10 ng/ml cholera toxin for 48 h. (A) 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) absorbance was measured and proliferation rate was calculated. (B and C) Fluorescent staining of nuclei by Hoechst 33258 (original magnification: ×400) in C6 cells treated with (C) or without (B) cholera toxin. (D) LDH absorbance in C6 cells. Results are means ± SD (n = 3). Statistical differences compared with the controls are given as *, P < 0.05 and **, P < 0.01.