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Up-regulation of CREB is required for cholera toxin-induced differentiation in C6 cells. (A) Western blot was used to estimate the levels of CREB after transfection with 20 nM siCREB for 48 h. (B and C) C6 cells were pretreated with siCREB for 48 h and then treated with 10 ng/ml cholera toxin for an additional 48 h for morphology (B) (original magnification: ×200) and Western blotting to evaluate GFAP and cyclin D1 (C).
