Figure 3.

An mRNA containing the entire 3′UTR of G-CSF is unstable in both NIH 3T3 and 5637 cells, and is not stabilized in NIH 3T3 cells treated with the calcium ionophore A23187. RNA was isolated at various times after serum stimulation of cells stably transfected with the fGH-GCSF gene and subjected to an RNase protection assay. The graphs show data as mean ± SEM from two experiments, and a representative RNase protection gel is shown with an arrow indicating the probe protected by fGH-GCSF and G indicating the glyceraldehyde-3-phosphate dehydrogenase internal standard.