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. 2007 Aug 15;2(8):e755. doi: 10.1371/journal.pone.0000755

Figure 4. Purified CD83Tg B cells display reduced Ig and increased IL-10 response to LPS stimulation.

Figure 4

Untouched B cells were isolated from pooled spleens of two 8–10 week old sex matched C57BL/6 (open squares) and CD83Tg (black squares) mice. 2×105 B cells were cultured in 5 replicates in the presence of LPS (10 µg/ml) or no further stimulus, as indicated on the x-axis. Proliferation (4A) or IL-10 secretion (4B) were measured after 48h. 4C: Total mouse Ig in the supernatant (dilution 1∶1024) was measured after seven days by ELISA. Each dot represents an individual experiment, employing B cells purified from two pooled spleens (mean of five replicates the SEM not shown, being below 10%). The bars indicate the median and the asterisks indicate a significant difference of the median (***p<0.0005), employing students t test.