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. 2007 Aug 15;2(8):e755. doi: 10.1371/journal.pone.0000755

Figure 6. Reduced IL-10 secretion by CD83mu B cells.

Figure 6

Untouched B cells were isolated from pooled spleens of two 8–10 week old sex matched C57BL/6 (open bars), CD83Tg (black bars) and CD83mu (dark grey bars) mice. 2×105 B cells were cultured in triplicates in the presence of titrated amounts of LPS as indicated on the x-axis. 6A: Total mouse Ig in the supernatant (dilution 1∶1024) was measured after seven days by ELISA. 6B: IL-10 in the supernatant was measured after 48 h. Presented are the combined results of 3 independent experiments, error bars show SEM. Asterisks indicate a significant difference of the mean (* p<0.05; ** p<0.005 *** p<0.0005) employing students t test.