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. 2007 Jun 27;2007:85154. doi: 10.1155/2007/85154

Figure 5.

Figure 5

Levels of IL-4 in the supernatants of spleen cells from rLicTXNPx or rLimTXNPx treated and untreated Balb/c mice. The spleen cells from untreated (a) and treated (b) Balb/c mice (50 μg of rLicTXNPx or rLimTXNPx i.p. injected once a week for 3 weeks followed by 2 weeks before the spleen cells were recovered) were incubated with rLicTXNPx or rLimTXNPx (10 μg/ml) in the presence or absence of ConA (5 μg/ml) for 48 hours. The levels of IL-4 were determined by ELISA in comparison with a standard curve using the recombinant IL-4. The data represent means and standard deviations for triplicate cultures of spleen cells from three mice. The results are from a representative experiment of three carried out independently. Statistical analysis was performed using Student t-test. Statistically, significant differences is indicated. *P < .05 and **P < .01.