Table 3.
Group means of clinical chemistry results of DIO-AKR mice. Terminal blood samples were obtained at the end of treatment. Serum levels of analytes were determined using an Olympus AU640 clinical chemistry analyzer and analyzed by a two-way analysis of variance with repeated measures model (ANOVA) followed by Dunnett's post hoc test. Values were considered to be significant (*) when the value of P < .05 was achieved.
| Treatment | Glucose (mg/dL) | Insulin (ng/mL) | Triglyceride (mg/dL) | NEFA (mEq/L) | Cholesterol (mg/dL) | HDL-C (mg/dL) | bHBA(mg/dL) | |
|
| ||||||||
| Experiment 1 | Lean vehicle | 211.3 ± 7.8 | 1.7 ± 1.4 | 194.7 ± 0.7 | 0.7 ± 0.03 | 89.5 ± 1.6 | 57.2 ± 1.1 | 2.1 ± 0.2 |
| DIO vehicle | 241.2 ± 7.4 | 11.2 ± 2.1 | 154.5 ± 8.1 | 0.8 ± 0.02 | 188.7 ± 12.5 | 131.8 ± 4.5 | 2.0 ± 0.2 | |
| GW9578 (1) | 248.0 ± 16.6 | 3.1 ± 1.6* | 168.3 ± 15.4 | 0.9 ± 0.04 | 117.1 ± 4.1 | 86.6 ± 2.7 | 3.2 ± 1.1 | |
| GW0742 (30) | 253.3 ± 8.9 | 2.2 ± 1.2* | 174.9 ± 10.3 | 0.9 ± 0.02 | 171.9 ± 2.9 | 118.4 ± 1.6 | 3.8 ± 0.3* | |
| GW9578 + GW0742 after week 2 | 211.5 ± 14.2 | 1.9 ± 0.9* | 97.5 ± 5.8* | 0.6 ± 0.02* | 207.5 ± 4.5* | 131.8 ± 4.5 | 7.0 ± 0.5* | |
| GW4148 + GW0742 after week 2 | 157.4 ± 2.4* | 2.9 ± 1.1* | 100.0 ± 8.9* | 0.6 ± 0.04* | 210.1 ± 8.5* | 132.5 ± 4.7 | 7.0 ± 1.4* | |
| GW9578 and GW0742 4 weeks | 199.1 ± 1.6* | 2.6 ± 0.8* | 128.1 ± 9.0* | 0.8 ± 0.04 | 234.4 ± 5.5* | 141.8 ± 2.3 | 6.9 ± 0.8* | |
|
| ||||||||
| Experiment 2 | DIO vehicle | 225.4 ± 11.7 | 10.2 ± 0.5 | 152.3 ± 0.8 | 0.7 ± 0.8 | 114.9 ± 2.9 | 81.8 ± 1.6 | 1.6 ± 0.2 |
| RIM (10) | 230.1 ± 16.6 | 7.2 ± 2.5* | 181.4 ± 36.1 | 0.8 ± 0.05 | 147.3 ± 9.1* | 95.1 ± 4.6* | 2.5 ± 0.2* | |
| RIM (30) | 215.3 ± 13.0 | 3.4 ± 0.7* | 159.7 ± 16.1 | 0.9 ± 0.02 | 143.4 ± 4.0* | 116.9 ± 2.6* | 1.8 ± 0.2 | |
| GW7845 (3) | 222.3 ± 9.4 | 2.1 ± 0.4* | 112.3 ± 5.7* | 0.6 ± 0.03* | 106.1 ± 4.2 | 65.0 ± 0.9* | 1.5 ± 0.1 | |
|
| ||||||||
| Experiment 3 | DIO vehicle | 234.0 ± 9.4 | 6.7 ± 1.9 | 163.5 ± 16.2 | 0.8 ± 0.07 | 116.1 ± 5.9 | 91.1 ± 3.7 | 2.9 ± 0.2 |
| GW4148 (3) | 233.3 ± 14.6 | 1.8 ± 0.3* | 62.6 ± 4.1* | 0.5 ± 0.03* | 178.9 ± 3.7* | 52.9 ± 0.1* | 5.1 ± 0.9* | |
| GW4148 (10) | 214.0 ± 10.9* | 1.3 ± 0.3* | 48.4 ± 3.6* | 0.5 ± 0.04* | 192.6 ± 6.8* | 131.1 ± 3.6* | 6.5 ± 1.1* | |
|
| ||||||||
| Experiment 4 | Lean vehicle | 189.5 ± 8.6 | 1.2 ± 0.2 | 220.5 ± 14.0 | 0.7 ± 0.04 | 70.0 ± 1.9 | 51.7 ± 1.4 | 1.6 ± 0.1 |
| DIO Vehicle | 196.0 ± 11.1 | 11.2 ± 2.1 | 277.6 ± 25.2 | 1.5 ± 0.10 | 122.8 ± 4.7 | 106.0 ± 2.8 | 3.7 ± 0.6 | |
| GW9135 (3) | 215.0 ± 14.1 | 2.9 ± 0.5* | 113.8 ± 9.9* | 0.8 ± 0.04* | 188.9 ± 4.9* | 149.4 ± 2.4* | 4.4 ± 0.6* | |
| GW9135 (10) | 196.1 ± 5.6 | 1.4 ± 0.4* | 58.8 ± 2.4* | 0.6 ± 0.02* | 183.8 ± 5.2* | 141.9 ± 2.8* | 4.3 ± 0.4* | |