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. Author manuscript; available in PMC: 2008 May 11.
Published in final edited form as: Neuroscience. 2007 Mar 23;146(2):741–755. doi: 10.1016/j.neuroscience.2007.01.056

Fig. 8.

Fig. 8

Effect of rotenone on activation of caspase-3 in cervical and lumbar SC. Representative photomicrographs of double immunofluorescent staining for active caspase-3 (red) and NeuN (green), performed in cervical and lumbar SC coronal slices (5 μm). No active caspase-3-IR was found in the sections from control animals. Significant amount of active caspase-3 was observed in many ventral motoneurons in cervical and lumbar SC segments of rotenone-injected animals, evaluated by increased co-localization of active caspase-3-IR and NeuN-IR (Merge indicate co-staining in rotenone panel). Images are taken at 200x magnification (n≥4).